Gambaran Infeksi Nontuberculous Mycobacteria (NTM) pada Penderita Suspek TB Paru Menggunakan Multiplex PCR (MPCR)-Universal Lateral Flow Assay (ULFA) Kit

What is Nontuberculous Mycobacteria (NTM) Disease?

www.thoracic.org C LI P A N D C O P Y What causes NTM disease? NTM comprise more than 160 different species of bacteria that are found naturally in the environment. The existing species (types) of NTM can vary from place to place in the world. The most common species causing disease is called Mycobacterium avium complex. The next most common are Mycobacterium abscessus complex and Mycobacterium...

Multiplex PCR and Reverse Line Blot Hybridization Assay (mPCR/RLB)

Multiplex PCR/Reverse Line Blot Hybridization assay allows the detection of up to 43 molecular targets in 43 samples using one multiplex PCR reaction followed by probe hybridization on a nylon membrane, which is re-usable. Probes are 5' amine modified to allow fixation to the membrane. Primers are 5' biotin modified which allows detection of hybridized PCR products using streptavidin-peroxidase...

Performance Evaluation of Anyplex plus MTB/NTM and AdvanSure TB/NTM for the Detection of Mycobacterium tuberculosis and Nontuberculous Mycobacteria

Background: Polymerase chain reaction (PCR) methods from direct specimen are widely used for the rapid and accurate detection of mycobacteria infection. In this study, we evaluated two domestically developed detection kits for Mycobacterium tuberculosis (MTB) and nontuberculous mycobacteria (NTM) using real-time PCR. Methods: A total of 348 samples from patients with suspected tuberculosis were...

[Evaluation of the performances of AdvanSure TB/NTM real time PCR kit for detection of mycobacteria in respiratory specimens].

BACKGROUND PCR is a widely used method for rapid and accurate diagnosis of mycobacteriosis. The sensitivity and specificity of a real time PCR kit newly developed in Korea were evaluated for detecting mycobacteria in respiratory specimens. METHODS One hundred twenty nine Mycobacterium tuberculosis (TB) culture positive respiratory specimens (82 AFB stain positive and 47 stain negative specime...

Does Disseminated Nontuberculous Mycobacterial Disease cause False-positive Determine TB-LAM Lateral Flow Assay Results?